L. Strandberg et al., THE USE OF FED-BATCH CULTIVATION FOR ACHIEVING HIGH CELL DENSITIES INTHE PRODUCTION OF A RECOMBINANT PROTEIN IN ESCHERICHIA-COLI, FEMS microbiology reviews, 14(1), 1994, pp. 53-56
The production of the fusion protein staphylococcal protein A/E. coli
beta-galactosidase in Escherichia coli was studied in batch and fed ba
tch cultivations. Batch cultivation of a recombinant E. coli strain yi
elded a final cell dry weight of 16.4 g l(-1) with a final intracellul
ar product concentration of recombinant protein corresponding to appro
ximately 38% of the cell dry weight. Fed batch cultivation made it pos
sible to increase the final cell dry weight to 77.0 g l(-1). The intra
cellular product concentration (25%) was lower as compared to batch cu
ltivation resulting in a total concentration of recombinant protein of
19.2 g l(-1).