A METHOD OF DEMONSTRATING INTRARENAL REFLUX

Objective To develop a method for demonstrating intrarenal reflux as a permanent resin cast, and subsequently to show the relationship betwe en the shape of the papilla and the presence and extent of reflux and to determine, by histological examination of the resin casts, the exte nt of filling of the nephron. Subjects and methods The pelvicaliceal s ystems of fresh pig's kidneys were tilled retrogradely with a dilute r esin that sets within 2 h. The parenchyma was then macerated in potass ium hydroxide to obtain the casts. Results The extent of the intra-ren al reflux was variable, was usually polar and appeared independent of the injection pressure. Microscopy showed branching of the collecting ducts and nephron filling to the proximal tubules. Conclusion This met hod could prove a useful tool in the further study of intra-renal refl ux.