SUPEROXIDE-DISMUTASE LEVEL IN RESPONSE TO PARAQUAT AND HIGH-TEMPERATURE IN THE CYANOBACTERIUM GLOEOCAPSA SP

Paraquat (methylviologen) at concentrations above 0.05 mM inhibited th e growth of photoautotrophic cyanobacterium Gloeocapsa sp. in axenic c ultures. The growth rate was not affected by concentrations of 0.01 mM or less. This concentration resulted after a lag period in a moderate increase in superoxide dismutase level. After removal of paraquat, th e cyanobacterium continued to generate higher levels of superoxide dis mutase. There was a lag period of one hour before resumption of normal enzyme activity. Addition of puromycin at concentration of 0.5 mg cm- 3 had no effect on cell survival, but greatly enhanced the sensitivity of the culture to the toxicity of paraquat. The data showed an increa se of SOD activity by temperatures above the normal growth temperature level. However, this increase was suppressed by chloramphenicol which revealed that the induction of superoxide dismutase by high temperatu res was associated with de novo protein synthesis.