Ma. Stackhouse et Js. Bedford, AN IONIZING RADIATION-SENSITIVE MUTANT OF CHO CELLS, IRS-20 .3. CHROMOSOME-ABERRATIONS, DNA BREAKS AND MITOTIC DELAY, International journal of radiation biology, 65(5), 1994, pp. 571-582
Citations number
72
Categorie Soggetti
Radiology,Nuclear Medicine & Medical Imaging","Nuclear Sciences & Tecnology
The ionizing radiation-sensitive mutant of CHO cells, irs-20, showed a
defect in the rate and extent of rejoining of gamma-ray-induced DNA d
ouble-strand breaks (dsbs) compared with the parental CHO cells as mea
sured using pulsed-field gel electrophoresis. Virtually all rejoined i
n wild-type cells but some 20-30% failed to rejoin in the mutant cells
during a 5-h period after irradiation. An increased level of chromoso
me-type aberrations per unit dose was seen in irs-20 cells compared wi
th wild-type cells irradiated during the G(1) phase. For the irs-20 ce
lls, about half the dose was required to produce the same chromosome-t
ype aberration frequency. Chromatid-type aberrations were induced in G
(1)-irradiated irs-20 cells at frequencies nearly the same as for chro
mosome types. For the parental wild-type CHO 10B2 cells, only chromoso
me types were seen. The distribution of aberrations among cells was no
t significantly different from Poisson for wild-type cells, but this w
as not the case for irs-20 cells where the overdispersion was highly s
ignificant. The mutant irs-20 cells displayed a much greater cell cycl
e delay per unit dose (about five-fold) in reaching mitosis after irra
diation in G(1) than the more radioresistant wild-type parental cells.