Pi. Akubue et al., EXCRETION OF MALONDIALDEHYDE, FORMALDEHYDE, ACETALDEHYDE, ACETONE ANDMETHYL ETHYL KETONE IN THE URINE OF RATS GIVEN AN ACUTE DOSE OF MALONDIALDEHYDE, Archives of toxicology, 68(5), 1994, pp. 338-341
A high pressure liquid chromatographic system (HPLC) has recently been
developed for the simultaneous detection of malondialdehyde (MDA), fo
rmaldehyde (FA), acetaldehyde (ACT) and acetone (ACON). We have examin
ed the urinary excretion of these four lipid metabolites in the urine
of rats following the acute oral administration of MDA, (158 mg/kg bod
y weight). During the first 12 h, increases in the urinary excretion o
f MDA and ACT of approximately 192- and 70-fold, respectively, were ob
served. The urinary excretion of both MDA and ACT decreased thereafter
. An increase in FA excretion was observed only 12-24 h after MDA admi
nistration. A significant decrease in ACON relative to control values
was observed 12-48 h after MDA treatment. Two new peaks were present i
n the HPLC chromatograms of urine samples 0-24 h after MDA administrat
ion. Both peaks were shown to be due to methyl ethyl ketone (MEK) whic
h appears to be formed as a result of MDA metabolism. The results demo
nstrate that orally administered MDA is rapidly excreted in the urine,
and alters the metabolism and excretion of other lipid metabolites.