THE SEQUENCE OF 29.7-KB FROM THE RIGHT ARM OF CHROMOSOME-II REVEALS 13 COMPLETE OPEN READING FRAMES, OF WHICH 10 CORRESPOND TO NEW GENES

We have determined the complete nucleotide sequence of a 29.7 kb segme nt from the right arm of chromosome II carried by the cosmid alpha 61. The sequence encodes the 3' region of the IRA1 gene and 13 complete o pen reading frames, of which ten correspond to new genes and three (CI F1, ATPsv and CKS1) have been sequenced previously. The density of pro tein coding sequences is particularly high and corresponds to 84% of t he total length. Two new genes encode membrane proteins, one of which is particularly large, 273 kDa. In one case (ATPsv), the comparison of our sequence and the published sequence reveals significant differenc es. The sequence has been deposited in the EMBL data library under the Accession Number X75891.