THE COMPLETE SEQUENCE OF A 33-KB FRAGMENT ON THE RIGHT ARM OF CHROMOSOME-II FROM SACCHAROMYCES-CEREVISIAE REVEALS 16 OPEN READING FRAMES, INCLUDING 10 NEW OPEN READING FRAMES, 5 PREVIOUSLY IDENTIFIED GENES ANDA HOMOLOG OF THE SCO1 GENE

We report here the sequence of a 33,117 bp DNA fragment located approx imately 30 kb from the centromere on the right arm of Saccharomyces ce revisiae chromosome II. We have detected 16 open reading frames (ORFs) longer than 450 bp, provisionally called YBR0301 to YBR0322, covering 70.4% of the entire sequence. The ORFs YBR0301, YBR0302, YBR0303, YBR 0305 and YBR0315 correspond to previously sequenced S. cerevisiae gene s GAL10, GAL1, FUR4, CAL1 and L2B, respectively. Translation products of two other ORFs, YBR0308 and YBR0312 exhibit similarity to previousl y known S. cerevisiae proteins: the mitochondrially associated protein SCO1 and the protein kinase YKR2. The predicted protein product of th e ORF YBR0321 shows a 41.6% identity score with the Escherichia coli p yroxamine 5'-phosphate oxidase. The nine other ORFs show no significan t homology to known proteins. The sequence has been deposited in the E MBL data library under Accession Number X76078.