SITE-SPECIFIC INCORPORATION OF PHOTOFUNCTIONAL NONNATURAL AMINO-ACIDSINTO A POLYPEPTIDE THROUGH IN-VITRO PROTEIN-BIOSYNTHESIS

Nonnatural amino acids with photofunctional groups were incorporated s ite-specifically into a polypeptide by using in vitro protein synthesi zing system. The nonnatural amino acids were attached to tRNA(CCU) thr ough chemical misacylation method, and added to the in vitro system wi th a mRNA containing a single AGG codon. L-p-Phenylazophenylalanine, L -2-anthrylalanine, L-1-naphthylalanine, L-2-naphthylalanine and L-p-bi phenylalanine were successfully incorporated into a polypeptide, but L -1-pyrenylalanine was not. The polypeptides containing the nonnatural amino acids showed photofunctionalities.