THE APPLICATION OF ELECTROSPRAY AND NEUTRAL-LOSS MASS-SPECTROMETRY TOTHE IDENTIFICATION OF THE METABOLITES OF S12813 IN RAT-LIVER SLICES

Authors
BROWNSILL R COMBAL JP TAYLOR A BERTRAND M LUIJTEN W WALTHER B
Citation
R. Brownsill et al., THE APPLICATION OF ELECTROSPRAY AND NEUTRAL-LOSS MASS-SPECTROMETRY TOTHE IDENTIFICATION OF THE METABOLITES OF S12813 IN RAT-LIVER SLICES, Rapid communications in mass spectrometry, 8(5), 1994, pp. 361-365
Citations number
2
Categorie Soggetti
Spectroscopy,"Chemistry Analytical
Journal title
Rapid communications in mass spectrometry → ACNP
ISSN journal
09514198
Volume
8
Issue
5
Year of publication
1994
Pages
361 - 365
Database
ISI
SICI code
0951-4198(1994)8:5<361:TAOEAN>2.0.ZU;2-G
Abstract
The metabolism of S12813, (3-{2-[4-phenyl piperazin-1-yl] ethyl}-2-oxo -2,3-dihydro oxazolo [4, 5-b] pyridine chlorohydrate), in rat liver sl ice incubates was examined by high performance liquid chromatography c ombined with mass spectrometry. Electrospray ionization was used toget her with tandem mass spectrometric techniques of analysis (MS/MS). Pol ar phase I and phase II metabolites were identified as C-oxidation pro ducts, which were then conjugated to form either sulphate or glucuroni de metabolites. On the basis of the identifications made, a metabolic pathway of S12813 in rat liver slices has been proposed.