CRYSTALLIZATION AT LOW-SALT CONCENTRATION AND ALKALINE PH AND PRELIMINARY CRYSTALLOGRAPHIC DATA FOR A MONOCLINIC FORM OF YEAST ENOLASE

Yeast enolase (2-phospho-D-glycerate hydrolyase, E.C. 4.2.1.11) has be en crystallized by vapor diffusion from a solution containing 22% PEG 4000, 100 mM Tris buffer pH = 9.3, 200 mM Li2SO4. The crystals are mon oclinic with a = 122.5, b = 111.8, c = 63.7 angstrom, beta = 95.6-degr ees, spa group P2(1) and two dimeric molecules are present in a asymme tric part of the unit cell. Crystals have been successfully transferre d to an artificial mother liquor, pH = 7.8, 20 mM in Mg2+ and 5 mM in 2-phospho-D-glycerate We believe that under these lower salt concentra tion an more alkaline conditions we should be able to localize th two metal ions that participate in catalysis as well a examine binding of high-affinity inhibitors.