FLUOROMETRIC MEASUREMENT OF THE ACTIVITY OF SURFACE-IMMOBILIZED MONOLAYERS OF ENZYME USING A DUAL EMISSION PROBE

Authors
BRENNAN JD KUKAVICA V KALLURY KMR KRULL UJ
Citation
Jd. Brennan et al., FLUOROMETRIC MEASUREMENT OF THE ACTIVITY OF SURFACE-IMMOBILIZED MONOLAYERS OF ENZYME USING A DUAL EMISSION PROBE, Canadian journal of chemistry, 72(3), 1994, pp. 721-728
Citations number
21
Categorie Soggetti
Chemistry
Journal title
Canadian journal of chemistry → ACNP
ISSN journal
00084042
Volume
72
Issue
3
Year of publication
1994
Pages
721 - 728
Database
ISI
SICI code
0008-4042(1994)72:3<721:FMOTAO>2.0.ZU;2-M
Abstract
A sensitive assay for measurement of the activity of hydrolase enzymes that are immobilized onto quartz and silicon dioxide surfaces was dev eloped. Measurements are based on the changes of the fluorescence prop erties of the probe carboxy-seminaptharhodafluor-X that are caused by pH variations associated with the reaction of urea with urease. The re sults indicated a loss of activity in 8 days, and a half-life of 3 day s, when urease was immobilized onto quartz surfaces that were first de rivatized with a 16-carbon spacer chain.