A novel method for evaluating pigment production during fermentation o
f Monascus ruber has been developed and consists of reflectance measur
ement with a 10-degrees observer under an artificial illuminant. A spe
ctrophotometer was adapted to measure pigmentation during culture in a
bioreactor. The lightness parameter L was the most useful information
delivered by this sensor. An absorbency over 30 units at 480nm on bat
ch cultures was estimated on-line without dilution.