EVIDENCE FOR HETEROGENEOUS TCR V-BETA REPERTOIRE EXPRESSION IN MERCURY-INDUCED IMMUNE DISORDERS IN RATS

Administration of subtoxic doses of HgCl2 affects differentially the i mmune system depending on the strain of rats tested, Susceptible Brown -Norway (BN) rats exhibit a CD4(+) T cell-dependent polyclonal activat ion of B cells; in contrast, Lewis (LEW) rats are resistant and develo p an immunosuppression mediated by CD8(+) T cells recruited by CD4(+) T cells. The mechanisms by which mercury induces immune disorders are poorly understood. We were interested in analyzing the diversity and m ercury-mediated changes of the TCR V-beta repertoire in the BN and LEW strains of rats at different times of HgCl2 exposure. Our results obt ained after analysis of lymph node T cells by RNase protection assay, flow cytometry or immunoscope assay (i) were not consistent with a sup erantigen-like stimulus since we observed neither a V-beta-selective e xpansion nor deletion that would have been expected and (ii) showed th at in BN rats, as well as in LEW rats, an increase in the number of T cells was associated with the heterogeneous TCR V-beta repertoire, thu s supporting a polyclonal T cell activation, However, in BN rats the t otal number of T cells increased very rapidly, whereas in LEW rats onl y CD8(+) T cells accumulated.