EXPRESSION OF GLUTATHIONE-S-TRANSFERASE ISOTYPES IN RELATION TO DOXORUBICIN SENSITIVITY AND PROGNOSIS IN PATIENTS WITH RENAL-CARCINOMA

Specimens were obtained from the tumour and adjacent normal kidney in 29 patients with renal carcinoma. Expression of the alpha, pi and mu i soforms of gluthatione-S-transferase (GST) was estimated in formalin f ixed paraffin embedded sections of the normal and neoplastic kidney fo llowing exposure to the appropriate polyclonal antibodies. binding of which was recognised by the indirect peroxidase technique. Expression of alpha, pi and mu GST in each tumour specimen was compared with the in vitro sensitivity of tumour cells to doxorubicin determined by drug inhibition of [Se-75] selenomethionine uptake by the appropriate tumo ur cells in culture. Expression of pi GST was associated with tumour c ell resistance to doxorubicin. The level of alpha, pi and mu GST was n ot related to patient survival.