SIMPLE AND RAPID METHOD FOR PURIFYING LOW-MOLECULAR-WEIGHT SUBUNITS OF GLUTENIN FROM WHEAT

Authors
MELAS V MOREL MH AUTRAN JC FEILLET P
Citation
V. Melas et al., SIMPLE AND RAPID METHOD FOR PURIFYING LOW-MOLECULAR-WEIGHT SUBUNITS OF GLUTENIN FROM WHEAT, Cereal chemistry, 71(3), 1994, pp. 234-237
Citations number
24
Categorie Soggetti
Food Science & Tenology","Chemistry Applied
Journal title
Cereal chemistry → ACNP
ISSN journal
00090352
Volume
71
Issue
3
Year of publication
1994
Pages
234 - 237
Database
ISI
SICI code
0009-0352(1994)71:3<234:SARMFP>2.0.ZU;2-L
Abstract
A method for the preparation of low molecular weight glutenin subunits (LMW-GS) of bread wheat without contamination by high molecular weigh t glutenin subunits (HMW-GS) or gliadins is described. Using a simple protocol based on the selective precipitation by acetone, two fraction s are obtained that correspond to HMW-GS and LMW-GS. The protein fract ions can be obtained either reduced or reduced and alkylated. The prot ocol can be scaled up to obtain large quantities of LMW-GS.