HEAT-SHOCK RESPONSE IN ACTINOBACILLUS-ACTINOMYCETEMCOMITANS

Citation
I. Lokensgard et al., HEAT-SHOCK RESPONSE IN ACTINOBACILLUS-ACTINOMYCETEMCOMITANS, FEMS immunology and medical microbiology, 8(4), 1994, pp. 321-328
Citations number
32
Categorie Soggetti
Immunology,Microbiology
ISSN journal
09288244
Volume
8
Issue
4
Year of publication
1994
Pages
321 - 328
Database
ISI
SICI code
0928-8244(1994)8:4<321:HRIA>2.0.ZU;2-P
Abstract
The heat shock response in Actinobacillus actinomycetemcomitans, a cap nophilic Gram-negative bacterial species that is implicated in the dev elopment of certain forms of periodontitis, was characterized. Differe nt strains of A. actinomycetemcomitans were grown at 37, 42 and 48 deg rees C in the presence of S-35-methionine. The bacterial cells were ly sed, run on SDS-PAGE and subsequently blotted on nitrocellulose paper. After autoradiography of the blots, several protein bands from the cu ltures at 42 degrees C showed an increased intensity; major bands were observed at 90, 70, and 60 kDa, but increased protein synthesis was a lso detected at 54, 28 and 17 kDa. Nitrocellulose blots were also incu bated with a panel of monoclonal and polyclonal antibodies directed to epitopes on different heat shock proteins. Strong reactivity was foun d with several antibodies at the position corresponding to a molecular mass of 60 kDa. The protein is probably the GroEL homologue in A. act inomycetemcomitans, a member of the 'common bacterial antigen' family.