STABILITY AND ACTIVITY OF HYDROGENASES OF METHANOBACTERIUM-THERMOAUTOTROPHICUM AND ALCALIGENES-EUTROPHUS IN REVERSED MICELLAR SYSTEMS

Authors
HOPPERT M BRAKS I MAYER F
Citation
M. Hoppert et al., STABILITY AND ACTIVITY OF HYDROGENASES OF METHANOBACTERIUM-THERMOAUTOTROPHICUM AND ALCALIGENES-EUTROPHUS IN REVERSED MICELLAR SYSTEMS, FEMS microbiology letters, 118(3), 1994, pp. 249-254
Citations number
20
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
118
Issue
3
Year of publication
1994
Pages
249 - 254
Database
ISI
SICI code
0378-1097(1994)118:3<249:SAAOHO>2.0.ZU;2-R
Abstract
In water-in-oil microemulsion the membrane-associated F-420-hydrogenas e of Methanobacterium themroautotrophicum (strain Marburg) and the mem brane-bound hydrogenase of Alcaligenes eufrophus H 16 (MBH) showed pro longed activity at elevated temperatures (measured as hydrogen product ion) as compared to aqueous buffer solution. The temperature optimum o f the reactions was about 15 degrees C higher than in aqueous buffer s olution. Activity of the almost completely inactivated F-420-hydrogena se could be partially recovered by transfer into microemulsion.