SEQUENCE-ANALYSIS OF COHESIVE ENDS OF THE ACTINOPHAGE RP3 GENOME AND CONSTRUCTION OF A TRANSDUCIBLE SHUTTLE VECTOR

Authors
KINNER E POCTA D STROER S SCHMIEGER HZ
Citation
E. Kinner et al., SEQUENCE-ANALYSIS OF COHESIVE ENDS OF THE ACTINOPHAGE RP3 GENOME AND CONSTRUCTION OF A TRANSDUCIBLE SHUTTLE VECTOR, FEMS microbiology letters, 118(3), 1994, pp. 283-289
Citations number
20
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
118
Issue
3
Year of publication
1994
Pages
283 - 289
Database
ISI
SICI code
0378-1097(1994)118:3<283:SOCEOT>2.0.ZU;2-M
Abstract
The sequence of the cohesive ends of actinophage RP3 DNA has been dete rmined. As with all other phages of Gram-positive bacteria that have b een studied sofar, RP3 DNA has 3'-protruding ends. A shuttle cosmid ha s been constructed containing this cas area which can be efficiently t ransduced by phage RP3 to host cells of Streptomyces rimosus.