ALLIANCES OF MUHLENBERGIA (POACEAE) WITHIN NEW-WORLD ERAGROSTIDEAE ARE IDENTIFIED BY PHYLOGENETIC ANALYSIS OF MAPPED RESTRICTION SITES FROMPLASTID DNAS

Restriction sites for six enzymes were mapped for the plastid DNAs of 25 species of Eragrostideae, one species of Cynodonteae (Eustachys dis tichophylla), and one species of Pooideae. Of the 124 restriction site s observed, 67 were variably present and shared by two or more species . These data were analyzed by the parsimony method using equal and une qual weights and by bootstrap analysis. The cladistic analyses establi shed that members of the Muhlenbergiinae, including the genera Muhlenb ergia, Blepharoneuron, Bealia. Chaboissaea, Lycurus, and Pereilema, sh are seven restriction site mutations and are strongly supported by the data as a monophyletic subtribe. Surprisingly, Redfieldia flexuosa al so clustered with the Muhlenbergiinae in the analysis, perhaps indicat ive of a past interspecific hybridization event. The restriction sites data also weakly support a relationship (six shared mutations) betwee n Erioneuron, Munroa, and Dasylochloa.