S. Dikanov et al., X-BAND ESEEM SPECTROSCOPY OF N-15 SUBSTITUTED NATIVE AND INHIBITOR-BOUND SUPEROXIDE-DISMUTASE - HYPERFINE COUPLINGS WITH REMOTE NITROGEN OFHISTIDINE LIGANDS, FEBS letters, 345(1), 1994, pp. 55-60
The hyperfine couplings of the remote nitrogens of histidine ligands a
re determined for the first time by an X-band ESEEM spectroscopy study
of N-15-substituted superoxide dismutase (SOD). They show a significa
nt difference between two groups of ligands with different orientation
relative to the metal ion. The ESEEM spectra of N-15 SOD With cyanide
as an inhibitor containing N-14 and N-15 are also discussed. They all
ow some conclusions to be drawn about structural changes upon inhibito
r binding and indicate the necessity of further multifrequency investi
gations.