AVAILABILITY OF FLUOROGENIC REAGENTS HAVING A BENZOFURAZAN STRUCTURE IN THE BIOSCIENCES

Fluorogenic reagents having a 2,1,3-benzoxadiazole (benzofurazan) moie ty are reviewed in terms of reactivity, fluorescence characteristics, sensitivity and applicability in the biosciences. Those included are 4 -fluoro-7-nitrobenzofurazan (NBD-F), ethylaminosulphonyl)-7-fluoro-2,1 ,3-benzoxadiazole (DBD-F), 4-aminosulphonyl-7-fluoro-2,1,3-benzoxadiaz ole (ABD-F), ammonium 7-fluoro-2,1,3-benzoxadiazole-4-sulphonate (SBD- F), 4-hydrazino-7-nitro-2,1,3-benzoxadiazole (NBD-H), 4-hydrazino-7-ni tro-2,1,3-benzoxadiazole hydrazine (NBD-H NH2NH2), ylaminosulphonyl)-7 -hydrazino-2,1,3-benzoxadiazole (DBD-H), 4-aminosulphonyl-7-hydrazino- 2,1,3-benzoxadiazole (ABD-H), 4-nitro-7-N-piperazino-2,1,3-benzoxadiaz ole (NBD-PZ), ro-7-(2-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole (D-NB D-APy), ro-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole (L-NBD-APy) , -dimethylamino-7-N-piperazino-2,1,3-benzoxadiazole (DBD-PZ), -dimeth ylamino-7-N-cadaverino-2,1,3-benzoxadiazole (DBD-CD), osulphonyl)-7-(1 -piperazynyl)-2,1,3-benzoxadiazole (ABD-PZ), honyl)-7-(5-aminopentylam ino)-2,1,3-benzoxadiazole (ABD-AP), inosulphonyl)-7-(2-aminoethyl)2,1, 3-benzoxadiazole (ABD-AE) and ammonium 7-N-piperazino-2,1,3-benzoxadia zole-4-sulphonate (SBD-P2). Their application data in liquid chromatog raphy are listed in tables. Future trends for the benzofurazan reagent s are discussed.