CONTINUOUS-FLOW CHEMILUMINOMETRIC DETERMINATION OF AMILORIDE AND STREPTOMYCIN BY OXIDATION WITH N-BROMOSUCCINIMIDE

A rapid continuous-flow method for the determination of 0.50-15.0 mu g ml(-1) of amiloride and 2.00-30.0 mu g ml(-1) of streptomycin is desc ribed. The method is based on the chemiluminogenic oxidation of the an alytes by N-bromosuccinimide in alkaline solution. The procedure is au tomated and solutions can be analysed at a rate of 250 h(-1) with a re lative error of about 1.6%. When applied to commercial formulations, t he procedure was relatively free from interferences from common excipi ents. The method is evaluated by a recovery study and by the analysis of commercial formulations. The results obtained for the assay of comm ercial pharmaceutical preparations compared well with those obtained b y the official methods and demonstrated good accuracy and precision.