INHIBITION OF IN-VITRO TRANSLATION BY ANTISENSE OLIGONUCLEOTIDES COVALENTLY-LINKED TO A NUCLEIC-ACID CLEAVER BASED ON A CATIONIC MANGANESE PORPHYRIN MOTIF, MN-TRISMPYP

Antisense oligonucleotides, modified by a anganese(III)tris(4-N-methyl pyridiniumyl)porphyrin (an artificial nuclease) and activated by an ox idant to promote their nuclease activity, exhibit a higher inhibitory effect on the in vitro translation of chloramphenicol acetyltransferas e (CAT) mRNA than the corresponding unmodified oligonucleotides, This effect is as high as that observed in the presence of E. Coli RNase H.