Sg. Bhagwat et Cr. Bhatia, QUANTITATIVE DIFFERENCES IN THE GIBBERELLIN INDUCED ALPHA-AMYLASE ACTIVITY FROM ALEURONE LAYERS OF TALL AND SEMIDWARF WHEAT CULTIVARS, Cereal Research Communications, 22(1-2), 1994, pp. 129-134
Aleurone layers isolated from mature grains of tall (Chinese Spring, N
P-876, C-273 and K-68) and semi-dwarf (Kalyansona and Moti) wheat cult
ivars were incubated under aseptic conditions in the presence or absen
ce of gibberellin (GA3). In presence of GA3 the aleurone layers synthe
sized and secreted large amounts of alpha-amylase into the medium betw
een 20-48h incubation. The semi-dwarfs carrying Rht1 and/Rht2genes sho
wed significantly less stimulation as compared to the talls. This tren
d was also observed in a time course study from 20 to 48h incubation.
This differential response of aleurone layers was also observed in a p
etri plate assay.