IDENTIFICATION OF AFRICAN HORSE SICKNESS VIRUS IN CELL-CULTURE USING A DIGOXIGENIN-LABELED RNA PROBE

Authors
BROWN CC MEYER RF GRUBMAN MJ
Citation
Cc. Brown et al., IDENTIFICATION OF AFRICAN HORSE SICKNESS VIRUS IN CELL-CULTURE USING A DIGOXIGENIN-LABELED RNA PROBE, Journal of veterinary diagnostic investigation, 6(2), 1994, pp. 153-155
Citations number
NO
Categorie Soggetti
Veterinary Sciences
Journal title
Journal of veterinary diagnostic investigation → ACNP
ISSN journal
10406387
Volume
6
Issue
2
Year of publication
1994
Pages
153 - 155
Database
ISI
SICI code
1040-6387(1994)6:2<153:IOAHSV>2.0.ZU;2-#
Abstract
A digoxigenin-labeled RNA probe was synthesized from a plasmid contain ing a portion of the African horse sickness virus (AHSV) serotype 4 ge nome segment coding for nonstructural protein 1. In an in situ hybridi zation procedure, this probe hybridized successfully to Vero cells inf ected with each of the 9 serotypes of AHSV. There was no hybridization with noninfected cell cultures or cell cultures infected with blueton gue virus.