THE EFFECT OF PHYTIC ACID ON THE RESOLUTION OF PEPTIDES AND PROTEINS IN CAPILLARY ELECTROPHORESIS

Authors
OKAFO GN BIRRELL HC GREENAWAY M HARAN M CAMILLERI P
Citation
Gn. Okafo et al., THE EFFECT OF PHYTIC ACID ON THE RESOLUTION OF PEPTIDES AND PROTEINS IN CAPILLARY ELECTROPHORESIS, Analytical biochemistry, 219(2), 1994, pp. 201-206
Citations number
14
Categorie Soggetti
Biology
Journal title
Analytical biochemistry → ACNP
ISSN journal
00032697
Volume
219
Issue
2
Year of publication
1994
Pages
201 - 206
Database
ISI
SICI code
0003-2697(1994)219:2<201:TEOPAO>2.0.ZU;2-X
Abstract
Studies are reported on the effect of the sodium salt of phytic acid o n the resolution of peptides and proteins. Improved separation in the case of peptides is shown to be due to ion-ion pairing interactions be tween the positively charged peptides and the phytic acid polyanionic species, The improved peak shapes related to the proteins can be inter preted in terms of the sample preconcentration due to injection of ana lytes from a water medium to one of high ionic strength. (C) 1994 Acad emic Press, Inc.