THE EFFECT OF HOMOGENATES AND EXCRETORY SECRETORY PRODUCTS OF NIPPOSTRONGYLUS-BRASILIENSIS AND OF ACETYLCHOLINESTERASE ON THE AMPLITUDE ANDFREQUENCY OF CONTRACTION OF UNINFECTED RAT INTESTINE IN-VITRO/
N. Foster et al., THE EFFECT OF HOMOGENATES AND EXCRETORY SECRETORY PRODUCTS OF NIPPOSTRONGYLUS-BRASILIENSIS AND OF ACETYLCHOLINESTERASE ON THE AMPLITUDE ANDFREQUENCY OF CONTRACTION OF UNINFECTED RAT INTESTINE IN-VITRO/, Parasitology, 108, 1994, pp. 453-459
Whole worm homogenates and excretory/secretory (E/S) products of adult
Nippostrongylus brasiliensis significantly decreased the amplitude of
contractions of segments of uninfected rat intestine maintained in an
organ bath, with significantly larger volumes of E/S products being r
equired to bring about a similar decrease to that caused by the whole
worm homogenate. Boiled samples of homogenate and E/S products signifi
cantly decreased the amplitude of contractions of uninfected rat intes
tine, but larger volumes were needed than with unboiled samples. Frequ
ency of contraction was unaltered by homogenates or E/S products. When
electric eel AChE was injected into the lumen of segments of uninfect
ed rat intestine maintained in an organ bath there was no significant
decrease in the amplitude of contractions. These results suggest that
substances present in the E/S products of N. brasiliensis significantl
y reduce the amplitude of contractions of uninfected rat intestine in
vitro and that the biochemical holdfast responsible for this phenomeno
n may not be AChE.