INHIBITION OF PHOSPHOLIPASE-D BLOCKS ACTIVATION OF FIBRINOGEN-ADHERENT NEUTROPHILS BY TUMOR-NECROSIS-FACTOR

Tumor necrosis factor stimulates fibrinogen-adherent neutrophils to pr oduce a dramatic oxidative burst; the resulting superoxide and other p roducts may contribute to tissue damage in severe infection. Inhibitor s of the phospholipase D pathway blocked this activation without affec ting cell adherence or nonspecific activation by phorbol myristate ace tate. The phospholipase D pathway appears to be involved in the activa tion of adherent neutrophils by tumor necrosis factor, and this pathwa y may be a target for modulation of tumor necrosis factor's effects.