K. Elmazouari et al., F-17-LIKE FIMBRIAE FROM AN INVASIVE ESCHERICHIA-COLI STRAIN PRODUCINGCYTOTOXIC NECROTIZING FACTOR TYPE-2 TOXIN, Infection and immunity, 62(6), 1994, pp. 2633-2638
The F17b fimbriae encoded by the transmissible virulence plasmid Vir,
also coding for cytotoxic necrotizing factor type 2, were characterize
d. A 5.7-kb region of Vir mediates in vitro N-acetylglucosamine-sensit
ive adhesion to calf intestinal villi. Sequence analysis revealed that
this region codes for a structural subunit and an adhesin closely rel
ated to the F17-A and F17-G proteins encoded by the F17 fimbrial gene
cluster. The F17b-A gene presents an open reading frame of 540 bp enco
ding a polypeptide of 180 amino acids with a putative signal peptide o
f 21 residues. The mature protein shows an identity of 74% with the F1
7-A structural subunit. This 20-kDa protein is recognized by antiserum
directed against F17 fimbriae. The F17b-G gene shows an open reading
frame of 1,029 bp encoding a polypeptide of 343 amino acids with a put
ative signal peptide of 22 residues. The F17b G polypeptide exhibits 9
5% identity with the F17-G adhesin. The functional homology of the gen
e products was further confirmed by demonstrating that mutants in the
F17-A gene can be complemented by the F17b-A gene and vice versa. Thes
e results prove that fimbriae belonging to the F17 family are also fou
nd on pathogenic Escherichia coli strains other than enterotoxigenic i
solates producing heat-labile or heat-stable enterotoxin.