IDENTIFICATION OF AN ESCHERICHIA-COLI PERIPLASMIC ACID-PHOSPHATASE CONTAINING OF A 27 KDA-POLYPEPTIDE COMPONENT

Authors
ROSSOLINI GM THALLER MC PEZZI R SATTA G
Citation
Gm. Rossolini et al., IDENTIFICATION OF AN ESCHERICHIA-COLI PERIPLASMIC ACID-PHOSPHATASE CONTAINING OF A 27 KDA-POLYPEPTIDE COMPONENT, FEMS microbiology letters, 118(1-2), 1994, pp. 167-173
Citations number
18
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
118
Issue
1-2
Year of publication
1994
Pages
167 - 173
Database
ISI
SICI code
0378-1097(1994)118:1-2<167:IOAEPA>2.0.ZU;2-M
Abstract
An acid phosphatase containing a 27-kDa polypeptide component has been identified in Escherichia cell by means of a zymogram technique. The enzyme is secreted in the periplasmic space and is able to hydrolyze s everal organic phosphate esters, but not diesters, showing preferentia l activity on p-nitrophenyl phosphate and other phenolic phosphate est ers. Production of the enzyme apparently occurs only in cells growing on carbon sources other than glucose.