Gm. Rossolini et al., IDENTIFICATION OF AN ESCHERICHIA-COLI PERIPLASMIC ACID-PHOSPHATASE CONTAINING OF A 27 KDA-POLYPEPTIDE COMPONENT, FEMS microbiology letters, 118(1-2), 1994, pp. 167-173
An acid phosphatase containing a 27-kDa polypeptide component has been
identified in Escherichia cell by means of a zymogram technique. The
enzyme is secreted in the periplasmic space and is able to hydrolyze s
everal organic phosphate esters, but not diesters, showing preferentia
l activity on p-nitrophenyl phosphate and other phenolic phosphate est
ers. Production of the enzyme apparently occurs only in cells growing
on carbon sources other than glucose.