TUMOR-NECROSIS-FACTOR-ALPHA UP-REGULATES TRANSFERRIN RECEPTORS IN K562 CELLS

Citation
M. Nezu et al., TUMOR-NECROSIS-FACTOR-ALPHA UP-REGULATES TRANSFERRIN RECEPTORS IN K562 CELLS, Journal of international medical research, 22(3), 1994, pp. 145-152
Citations number
22
Categorie Soggetti
Pharmacology & Pharmacy","Medicine, Research & Experimental
ISSN journal
03000605
Volume
22
Issue
3
Year of publication
1994
Pages
145 - 152
Database
ISI
SICI code
0300-0605(1994)22:3<145:TUTRIK>2.0.ZU;2-U
Abstract
The effects of tumour necrosis factor-alpha on transferrin receptor ex pression in a human chronic myelocytic leukaemia cell line, K 562 cell s, were studied. Cytofluorometry studies showed that the numbers of tr ansferrin receptors in exponentially growing K 562 cells were increase d when the cells were incubated with tumour necrosis factor-alpha for 24 h. The induction of transferrin receptors by tumour necrosis factor -alpha may be mediated by a mechanism that is independent of growth si nce cell growth in treated cultures did not differ from that in the co ntrols. The DNA contents of K 562 cells treated with tumour necrosis f actor-alpha showed that after 24 h there were less cells in the G1 and S phases and more cells in the G2/M phase than in the control group. The phase of upregulation of transferrin receptors induced by tumour n ecrosis factor-alpha may be dependent on the cell cycle. This new evid ence that tumour necrosis factor-alpha upregulates transferrin recepto rs suggests a cancer-anaemia cascade in which the cancer burden state activates macrophage release of tumour necrosis factor-alpha as a resu lt of transferrin receptor expression.