COMPARISON OF THE USE OF SECRETORY AND SOMATIC ANTIGENS IN AN ELISA FOR THE SERODIAGNOSIS OF HYPODERMOSIS

Authors
MARTINEZMORENO FJ WASSALL DA BECERRAMARTELL C HERNANDEZRODRIGUEZ S
Citation
Fj. Martinezmoreno et al., COMPARISON OF THE USE OF SECRETORY AND SOMATIC ANTIGENS IN AN ELISA FOR THE SERODIAGNOSIS OF HYPODERMOSIS, Veterinary parasitology, 52(3-4), 1994, pp. 321-329
Citations number
16
Categorie Soggetti
Parasitiology,"Veterinary Sciences
Journal title
Veterinary parasitology → ACNP
ISSN journal
03044017
Volume
52
Issue
3-4
Year of publication
1994
Pages
321 - 329
Database
ISI
SICI code
0304-4017(1994)52:3-4<321:COTUOS>2.0.ZU;2-1
Abstract
An antigen was prepared from metabolic products which were produced by maintaining first instar larvae of Hypoderma lineatum in RPMI tissue culture medium for 48 h. Three major proteins were identified in the s ecretory products and were characterised in terms of their molecular w eights and iso-electric points. The antigen compared favourably with a soluble extract of larvae when used in an enzyme-linked immunosorbent assay (ELISA) against a panel of control sera and 2000 bovine sera co llected from farms in Andalucia, Spain.