K. Lala et P. Narayanan, PURIFICATION, N-TERMINAL SEQUENCE AND STRUCTURAL CHARACTERIZATION OF A TOXIC PROTEIN FROM THE INDIAN SCORPION-VENOM BUTHUS-TAMULUS, Toxicon, 32(3), 1994, pp. 325-338
A toxic protein component (Bt-II) was isolated from the venom of the I
ndian scorpion, Buthus tamulus, by ion-exchange chromatography on Bior
ex-70 and reverse phase high pressure liquid chromatography (RP-HPLC).
This 7800 mol. wt protein was found to have an LD50 value of 2.25 mg/
kg body weight in mice by subcutaneous injection. Amino acid compositi
on of the protein revealed the presence of aromatic and basic amino ac
ids; the N-terminal amino acid sequence was also determined. Structura
l characterization of Bt-II, based on circular dichroic and fluorescen
t quenching studies, has also been reported.