OLIGO[D(C)CENTER-DOT(G)] RUNS EXHIBIT-A HELICAL REPEAT OF 11.1 BP IN SOLUTION AND CAUSE SLIGHT DNA CURVATURE WHEN PROPERLY PHASED

Citation
M. Biburger et al., OLIGO[D(C)CENTER-DOT(G)] RUNS EXHIBIT-A HELICAL REPEAT OF 11.1 BP IN SOLUTION AND CAUSE SLIGHT DNA CURVATURE WHEN PROPERLY PHASED, Nucleic acids research, 22(9), 1994, pp. 1562-1566
Citations number
38
Categorie Soggetti
Biology
Journal title
ISSN journal
03051048
Volume
22
Issue
9
Year of publication
1994
Pages
1562 - 1566
Database
ISI
SICI code
0305-1048(1994)22:9<1562:OREHRO>2.0.ZU;2-L
Abstract
We have insertedd d(C)(10) in a set of DNA fragments with bent segment s on both ends, which are rotated with respect to each other by base p air wise increasing insertions. The electrophoretic mobilities on poly acrylamide gels of these DNA fragments were used to identify insertion sizes with cis conformations of the bent ends. These experiments reve aled a helical repeat in solution of d(C).d(G) tracts of 11.1 +/- 0.08 bp. The electrophoretic mobilities of ligation ladders with properly phased d(C)(5) and d(C)(16) runs demonstrate a small but clearly detec table curvature of these fragments.