M. Biburger et al., OLIGO[D(C)CENTER-DOT(G)] RUNS EXHIBIT-A HELICAL REPEAT OF 11.1 BP IN SOLUTION AND CAUSE SLIGHT DNA CURVATURE WHEN PROPERLY PHASED, Nucleic acids research, 22(9), 1994, pp. 1562-1566
We have insertedd d(C)(10) in a set of DNA fragments with bent segment
s on both ends, which are rotated with respect to each other by base p
air wise increasing insertions. The electrophoretic mobilities on poly
acrylamide gels of these DNA fragments were used to identify insertion
sizes with cis conformations of the bent ends. These experiments reve
aled a helical repeat in solution of d(C).d(G) tracts of 11.1 +/- 0.08
bp. The electrophoretic mobilities of ligation ladders with properly
phased d(C)(5) and d(C)(16) runs demonstrate a small but clearly detec
table curvature of these fragments.