PURIFICATION OF A MAIZE DEHYDRIN

A maize dehydrin with an apparent molecular weight of 20 kDa was purif ied from whole kernels of maize inbred line G50. Kernels were ground i n a seed mill, stirred overnight in extraction buffer, and centrifuged to extract soluble proteins. The sample was heated to 89-degrees-C an d centrifuged to remove heat-insoluble proteins. The remaining soluble proteins were fractionated in a three-step chromatographic process. F ollowing cation exchange, hydrophobic interaction, and gel filtration chromatography, pure dehydrin samples were obtained. (C) 1994 Academic Press, Inc.