INHIBITION OF HIGHLY METASTATIC FBJ-LL CELL-MIGRATION BY GANGLIOSIDE GD1A HIGHLY EXPRESSED IN POORLY METASTATIC FBJ-S1 CELLS

For clarification of the functions of gangliosides on tumor metastasis , examination was made of the ganglioside patterns of poorly metastati c FBJ-S1 and highly metastatic FBJ-LL cells, FBJ-S1 cells expressed GM 3 and GD1a, whereas FBJ-LL cells expressed GM3 and slightly expressed GD1a. The capacity for FBJ-LL cells to migrate was ten times that of F BJ-S1 cells, but decreased by a half by pretreatment with GD1a. GD1b o r GT1b had the same effect as GD1a, and synthetic sialyl compounds to a lesser extent, suggesting that gangliosides contain more than two si alyl residues to inhibit the migration of FBJ-LL cells. (C) 1997 Acade mic Press.