MOUSE P150(GLUED) (DYNACTIN-1) CDNA SEQUENCE AND EVALUATION AS A CANDIDATE FOR THE NEUROMUSCULAR DISEASE MUTATION MND2

p150(Glued) (Dynactin 1) is a component of the dynactin complex that i s essential for retrograde axonal transport in neurons. The mouse p150 (Glued) cDNA was isolated from mouse brain by RT-PCR. The complete seq uence of the full length mouse cDNA was determined, including 19 bp of 5' UTR, 3843 bp of coding sequence, and 162 bp of 3' UTR. The predict ed protein has a molecular mass of 142 kDa and exhibits 95% amino acid sequence identity to the predicted amino acid sequence of human p150( Glued) (DCTN1). The mouse Dctn1 gene was previously mapped in the cent ral region of mouse chromosome 6, close to the neuromuscular disease g ene mnd2. Northern blot analysis, complete sequencing of the cDNA, Wes tern blot, and functional tests of the protein did not detect any abno rmalities of p150(Glued) in mnd2 mice. (C) 1997 Academic Press.