Jaf. Robledo et al., MONOCLONAL-ANTIBODIES AGAINST SPORANGIA AND SPORES OF MARTEILIA SP (PROTOZOA, ASCETOSPORA), Diseases of aquatic organisms, 18(3), 1994, pp. 211-216
Digestive glands of mussels Mytilus edulis from Brittany, France, infe
cted with Marteilia sp. (Ascetospora) were used to purify the parasite
. A modification of a previously used purification protocol increased
purification efficiency, permitting sporangial primordia and sporangia
of Marteilia sp. to be obtained. Mouse (Balb/c) monoclonal antibodies
were generated against this parasite. From the fusion, 26 monoclonal
antibodies against Marteilia sp. were obtained. Antibodies from 6 clon
es reacted only with Marteilia sp. cells and not with normal host tiss
ues. Four of these antibodies (1/1-3, 3/1-1, 4/1-1 and 6/2-3) reacted
with the sporangia wall and two with the spore cytoplasm (9/1-1 and 12
/5-1). Antibodies cross-reacted with Marteilia refringens from Mytilus
galloprovincialis obtained in the Ria de Vigo, Spain.