EFFECT OF (2,6-0-DIMETHYL) BETA-CYCLODEXTRIN (HEPTAKIS) ON CELL MASS,PERTUSSIS TOXIN AND FILAMENTOUS HEMAGGLUTININ PRODUCTION IN 3 BORDETELLA-PERTUSSIS VACCINAL STRAINS
Bnp. Derossi et al., EFFECT OF (2,6-0-DIMETHYL) BETA-CYCLODEXTRIN (HEPTAKIS) ON CELL MASS,PERTUSSIS TOXIN AND FILAMENTOUS HEMAGGLUTININ PRODUCTION IN 3 BORDETELLA-PERTUSSIS VACCINAL STRAINS, Revista de Microbiologia, 25(1), 1994, pp. 16-23
We have studied the production of pertussis toxin (PT) and filamentous
hemagglutinin (FHA) by three Bordetella pertussis vaccinal strains in
culture supernatants and in supernatants of cells washed with high io
nic strenght solutions. The strains were grown in Stainer-Scholte (SS)
medium and in a modification of it that we called CL-basal (CL-b) and
in these media with heptakis (2,6-0-Dimethyl beta-cyclodextrin (Mebet
aCD)). FHA and PT were estimated by total hemagglutination, differenti
al hemagglutination (with cholesterol) and Dot-blot immunoassay. CL-b
medium increase the cellular mass production and would also increase t
he stability and liberation of hemagglutinins. The addition of MebetaC
D to SS or CL-b medium would stimulate the production, exportation and
stability of PT and FHA. These effects are greater for CL-b medium an
d more important for FHA than for PT. We obtained similar results with
the addition of 0,5 or 1 g/l of MebetaCD to CL-b. Culture supernatant
s contained FHA and PT but supernatants of cells washed with high ioni
c strength solutions contained basically FHA and would be used to impr
ove its yield.