In vitro inhibition of the of spores germination of Alternaria solani
by iprodione, chlorothalonil, and anilazine at different dosages was s
tudied. The highest concentration of active ingredient studied for eac
h fungicide was equivalent to that recommended for the control of the
early blight, under field conditions: 0.75; 1.80 and 1.44 g, respectiv
ely, of iprodione, chlorothalonil and anilazine per litre of water. A
series of two-fold diluitions of each original concentration was studi
ed in additional nine experiments. Eah of the three fungicides showed
total in vitro spore inhibition at the highest rate, at six hours of i
ncubation. At nine hours, only analazine mantained its full inhibition
activity. The inhibition activity of iprodione decreased suddenly aft
er 1/2 dilution, so that at the 1/8 dilution a total loss of inhibitor
y activity was observed. Chlorothalonil showed a progressive and sligh
ter decrease of its activity as the dilution rate increased. Analizine
showed a high inhibitory activity at higher dilutions, without any lo
ss up to 1/128 dilution. Even at 1/512 dilution, its activity was so h
igh that only 20% of spore germination was observed at six or nine hou
rs of incubation.