AMPEROMETRIC BIOSENSOR FOR ADENOSINE-5'-TRIPHOSPHATE BASED ON A PLATINUM-DISPERSED CARBON-PASTE ENZYME ELECTRODE

A new amperometric biosensor for adenosine-5'-triphosphate (ATP) was d esigned using a platinum-dispersed carbon paste into which glycerol ki nase and glycerol-3-phosphate oxidase were incorporated. The biosensor is based on the detection of hydrogen peroxide produced by the enzyma tic reaction of ATP with glycerol and the subsequent oxidation of glyc erol-3-phosphate. The use of the platinum-dispersed carbon paste elect rode lowered the oxidation potential for hydrogen peroxide, permitting the sensitive detection of ATP at 0.4 V vs. Ag/AgCl. A linear respons e to ATP was observed in the concentration range of I x 10(-5) to 2.5 x 10(-3) M.