Ds. Na et al., EVANS BLUE STAINING METHOD FOR DETECTION OF PROTEINS ON POLYACRYLAMIDE GELS WITH RHODAMINE-B, Analytical letters, 27(7), 1994, pp. 1265-1275
A new staining method employing a mixed dye technique is simple, rapid
, and sensitive. Solutions of Evans blue (EB, 0.01 %) and rhodamine B
(RB, 0.5 %) were mixed up with 1:1 volume ratio. This mixed dye staini
ng method detects as little as 10 ng of bovine serum albumine (BSA). E
B is used for protein staining based on its structural similarity to C
oomassie brilliant blue R-250 (CB). RB acts as a supplementary agent w
hich inhibits the binding of EB to gel matrix, and so increases the st
aining effect of EB and enables to achieve cutting down staining/desta
ining times.