EVANS BLUE STAINING METHOD FOR DETECTION OF PROTEINS ON POLYACRYLAMIDE GELS WITH RHODAMINE-B

A new staining method employing a mixed dye technique is simple, rapid , and sensitive. Solutions of Evans blue (EB, 0.01 %) and rhodamine B (RB, 0.5 %) were mixed up with 1:1 volume ratio. This mixed dye staini ng method detects as little as 10 ng of bovine serum albumine (BSA). E B is used for protein staining based on its structural similarity to C oomassie brilliant blue R-250 (CB). RB acts as a supplementary agent w hich inhibits the binding of EB to gel matrix, and so increases the st aining effect of EB and enables to achieve cutting down staining/desta ining times.