N-PENT-4-ENOYL (PNT) GROUP AS A UNIVERSAL NUCLEOBASE PROTECTOR - APPLICATIONS IN THE RAPID AND FACILE SYNTHESIS OF OLIGONUCLEOTIDES, ANALOGS, AND CONJUGATES

Authors
IYER RP DONG Y HABUS I HO NH JOHNSON S DEVLIN T JIANG ZW WEN Z JIN X AGRAWAL S
Citation
Rp. Iyer et al., N-PENT-4-ENOYL (PNT) GROUP AS A UNIVERSAL NUCLEOBASE PROTECTOR - APPLICATIONS IN THE RAPID AND FACILE SYNTHESIS OF OLIGONUCLEOTIDES, ANALOGS, AND CONJUGATES, Tetrahedron, 53(8), 1997, pp. 2731-2750
Citations number
54
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Tetrahedron → ACNP
ISSN journal
00404020
Volume
53
Issue
8
Year of publication
1997
Pages
2731 - 2750
Database
ISI
SICI code
0040-4020(1997)53:8<2731:N(GAAU>2.0.ZU;2-V
Abstract
Rapid synthesis of phosphoric diester and phosphorothioate oligonucleo tides in 1 micromol to 1 millimol scale has been achieved using PNT nu cleoside phosphoramidites. Furthermore, facile synthesis of mixed back bone oligonucleotides (MBOs) as antisense agents can be carried out us ing PNT nucleoside phosphoramidites in conjunction with PNT nucleoside H-phosphonates and PNT nucleoside phosphonamidites. The versatility o f the PNT group is further demonstrated by its use in the preparation of bioreversible oligonucleotide conjugates. (C) 1997 Elsevier Science Ltd.