INDUCTION OF YEAST PSORULATION BY LYSINE-RELATED COMPOUNDS AND GLUTATHIONE IN NUTRITION-RICH CONDITIONS

In addition to DL-lysine (Kawado et al., J. Ferment. Bioeng., 76, 391- 394, 1993), DL-2-aminoadipate was found to induce sporulation in a wil d-type yeast of Saccharomyces cerevisiae 4011 growing in a nutrition-r ich medium without potassium acetate. In the presence of the acid at 1 0 mM, the sporulation frequency of the yeast cells increased in parall el with the growth and reached maximum (26%) in the stationary phase. Despite the expression of a meiosis inducer gene, IME1, no sporulation was induced unless DL-lysine or DL-2-aminoadipate was included in the nutrition-rich medium. By using DL-2-aminoadipate in combination with glutathione, sporulation was also induced in industrially used sake y easts growing in the nutrition-rich medium without-potassium acetate.