Hydroxytyrosol, a polyphenolic compound of chemical and biological int
erest, present as the main polyphenol in olive oil vegetation waters (
VW), was purified from VW by means of a chromatographic method based o
n three steps. The compound was identified by UV, IR, H-1- and C-13-NM
R and EIMS. Its preparation from a natural source, as VW, for laborato
ry use (analytical and biological studies, for example) is convenient
from an economic and ecological point of view. The compound, labile in
air and light, was transformed into the more stable triacetyl derivat
ive, which was characterized by spectroscopic analysis, using the tech
niques mentioned above.