EVIDENCE FOR A SITE-SPECIFIC ENDONUCLEASE IN YEAST MITOCHONDRIA WHICHRECOGNIZES THE SEQUENCE 5'GCCGGC

Authors
EZEKIEL UR ZASSENHAUS HP
Citation
Ur. Ezekiel et Hp. Zassenhaus, EVIDENCE FOR A SITE-SPECIFIC ENDONUCLEASE IN YEAST MITOCHONDRIA WHICHRECOGNIZES THE SEQUENCE 5'GCCGGC, Biochemical and biophysical research communications, 201(1), 1994, pp. 208-214
Citations number
11
Categorie Soggetti
Biology,Biophysics
Journal title
Biochemical and biophysical research communications → ACNP
ISSN journal
0006291X
Volume
201
Issue
1
Year of publication
1994
Pages
208 - 214
Database
ISI
SICI code
0006-291X(1994)201:1<208:EFASEI>2.0.ZU;2-O
Abstract
We have discovered a mitochondrial, site-specific DNase in Saccharomyc es cerevisiae with properties like that of a Type II restriction endon uclease. The enzyme, termed Scelll, cleaves the palindromic sequence, 5'GCCGGC, to give 3' ends recessed by 4 bases. Scelll is the first res triction-like endonuclease to be described in yeast mitochondria. (C) 1994 Academic Press, Inc.