CLONING AND CHARACTERIZATION OF THE HUMAN THROMBOXANE SYNTHASE GENE PROMOTER

A genomic phage clone hybridized to the 5' end of human thromboxane sy nthase (TS) cDNA was isolated. Sequencing analysis of a 1.7 kb subfrag ment revealed that it contained the entire 5' untranslated region and 46 bp of the coding sequence of TS cDNA, an upstream canonical TATA bo x (TATAAA), and several binding sites for transcription factors (AP1, PEA-3, PU.1, and GR), indicative of a promoter/first exon region of th e TS gene. RNase protection assay mapped the transcription start site of the human TS gene to the nucleotide A 30 bp downstream from the TAT A box. The authenticity of the promoter was further confirmed by its a bility to direct expression of a CAT reporter gene in transfected HL60 cells. (C) 1994 Academic Press, Inc.