DELETION MAPPING OF CHROMOSOME-19 IN HUMAN GLIOMAS

There is evidence that a putative glioma tumor suppressor locus reside s on the long arm of chromosome 19. We present data on 161 gliomas fro m 156 patients, which were studied by microsatellite analysis for loss of heterozygosity (LOH) on chromosome 19. Eight loci on the long arm and 2 loci on the short arm of chromosome 19 were examined. LOH on 19q was observed in 3/19 astrocytomas (WHO grade II), 12/27 anaplastic as trocytomas (WHO grade III), 16/76 cases of glioblastoma multiforme WHO (grade IV), 4/9 oligodendrogliomas (WHO grade II), 3/5 anaplastic oli godendrogliomas (WHO grade III), 5/9 mixed oligo-astrocytomas (WHO gra de II) and 8/10 anaplastic oligo-astrocytomas (WHO grade III). While 3 1 of the tumors with LOH on chromosomal arm 19q exhibited allelic loss at every informative locus, 20 tumors showed terminal or interstitial deletions. In contrast to astrocytomas and glioblastomas, tumors with an oligodendroglial component had predominantly lost the entire long arm of chromosome 19. The common region of overlap in gliomas was loca ted on 19q13.2-q13.4 between the markers D19S178 and D19S180. Our data confirm the involvement of a putative tumor suppressor gene on chromo somal arm 19q in gliomas and assign this gene to 19q13.2-q13.4. (C) 19 94 Wiley-Liss, Inc.