ADSORPTION OF IMMUNOGLOBULIN-G AND ANTI-FACTOR-VIII INHIBITORY ANTIBODY FROM HEMOPHILIAC PLASMA TO DERIVATIZED POLYSTYRENES

Modified polystyrene resins containing sulphonate groups and tyrosyl s ulphamide or tyrosyl methyl ester sulphamide groups have been investig ated with respect to their potential for selective binding of anti-fac tor VIII inhibitory antibodies from plasma. Adsorption of total immuno globulin G and of a monoclonal antibody to Factor VIII was measured fo llowing addition of the radioiodinated proteins to normal plasma, plas ma depleted of Factor VIII by adsorption on a resin coupled to anti-fa ctor VIII antibody, and haemophiliac plasma containing various levels of inhibitory anti-factor VIII antibody. Depletion of anti-factor VIII antibody from the haemophiliac plasmas by incubation with the resins was also measured by Bethesda assay. The modified resins and their cor responding unmodified 'controls' showed similar binding of total immun oglobulin G. However, only resins containing either sulphonate or a co mbination of sulphonate and tyrosyl sulphamide groups showed evidence of selective adsorption of anti-factor VIII antibody from plasma. (C) 1997 Elsevier Science Limited. All rights reserved.