RESOLUTION OF AHYDRO-1-METHYL-1H-AZEPIN-4YL)-1(2H)-PHTHALAZINONE ENANTIOMERS IN PLASMA WITH FRIT-FAB LC MS USING A CONALBUMIN COLUMN

A new column-switching method for analysis of drug enantiomers in plas ma has been developed with liquid chromatography-frit fast atom bombar dment mass spectrometry in combination with a chiral resolution column , which consists of conalbumin (egg-white glycoprotein) immobilized on silica gel and can be used in the reversed-phase separation mode. Thi s method makes it possible to inject a large volume of deproteinized p lasma and obtain resolution of drug enantiomers with high sensitivity. The optimum mobile phase, including a non-volatile buffer such as pho sphate buffer, for separation of drugs from a large amount of endogeno us compounds can be used, because of inclusion of a trapping column wi th a desalting function. This method is very simple and rapid, and sho uld be very powerful in studies requiring high-sensitivity analysis wi th chiral separation of drugs from biological samples such as plasma.